In this work, pancreatic lipase was immobilized on 3D-printed PLA microwells for enzyme inhibitor screening.
Thus, designing and fabricating scaffolds on a similar scale, which could serve as immobilization carriers, and their recruitment in inhibitors screening studies is of great significance, adding both enzyme stability and reuse potentiality of the biocatalytic system in assay merits. Hence, assays based on microtiter plates are predominant. The prerequisites for an efficient screening assay include using small volumes of reagents, low cost, and rapid screening of large numbers of compounds and extracts. This is an open access article distributed under the conditions of the Creative Commons by Attribution License, which permits unrestricted use, distribution, and reproduction in any medium or format, provided the original work is correctly cited.ģD printed PLA has already been demonstrated for several biotechnological applications, including enzymes immobilization. 3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase. Recommended citation: Skonta A, Gkantzou E, Spyrou K, Spyrou S, Polydera A, Gournis D, Stamatis H. * Correspondences: Elena Gkantzou and Haralambos StamatisĪcademic Editor: Agnieszka Kolodziejczak Radzimska Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greeceĭepartment of Materials Science and Engineering, University of Ioannina, Ioannina, Greece Authors should present their results in as much detail as possible, as reviewers are encouraged to emphasize scientific rigor and reproducibility.Īnastasia Skonta 1, Elena Gkantzou 1,*, Konstantinos Spyrou 2, Stamatia Spyrou 1, Angeliki Polydera 1, Dimitrios Gournis 2, Haralambos Stamatis 1,* There is no restriction on paper length, provided that the text is concise and comprehensive. It publishes a variety of article types: original research, review, communication, opinion, study protocol, comment, conference report, technical note, book review, etc. Organocatalysis, catalysis in organic and polymer chemistry.Biocatalysis, enzymes, enzyme catalysis.Its aim is to provide timely, authoritative introductions to current thinking, developments and research in carefully selected topics. This periodical is devoted to publishing high-quality papers that describe the most significant and cutting-edge research in all areas of catalysts and catalyzed reactions. Catalysis Research is an international peer-reviewed Open Access journal published quarterly online by LIDSEN Publishing Inc.
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